Skip to main content
. 2016 Nov 18;17(11):1928. doi: 10.3390/ijms17111928

Figure 5.

Figure 5

p53 as the apoptotic executor of TSN. (A,B) U87 cells were transfected with siRNA targeting p53 and treated with 10 nM TSN for an additional 48 h. The expression of Bcl-2, Bax, and pAkt and flow cytometry were evaluated to determine apoptosis. siRNA against GFP was used as a control for the transfection procedure; (C,D) The T98G/ERβ cells expressing M237I-mutated p53 were transfected with either wild-type p53 plasmid or empty vector (EV), treated with 10 nM TSN, and evaluated for apoptosis by Western blot and flow cytometry.