Figure 4.

(a) Protein expression of cytoplasmic and mitochondrial cytochrome c detected by western blot and the numbers below every picture represent semi-quantitative analysis of each line; (b,c) Relative OD₄₀₅ value of U2OS cells treated with different concentrations (b) and different time points (c) of oleandrin, relative to the corresponding controls; n = 3, Mean ± SEM; and (d,e) Relative OD₄₀₅ value of SaOS-2 cells treated with different concentrations (d) and different time points (e) of oleandrin, relative to the corresponding controls; n = 3, Mean ± SEM.