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(a) Apoptosis changes of cells that pre-treated with z-VAD-fmk followed by oleandrin (50 nM) addition that detected by FCM and (b) Protein expression of cleaved caspase-9, cleaved caspase-8 and cleaved caspase-3 in U2OS cells detected by western blot after pre-treating with z-LEHD-fmk or a Fas blocking antibody. The numbers below every picture represent semi-quantitative analysis of each line.
