Skip to main content
. 2016 Nov 23;17(11):1961. doi: 10.3390/ijms17111961

Figure 8.

Figure 8

RAGE silencing reduces closure of an in vitro scratch wound, gingival epithelial cell migration and PCNA expression. (A) Representative photomicrographs of a cell migration assay. Cells were pretreated with 300 nM siRNA against RAGE and stimulated with rHMGB1 at different concentrations in culture media containing 2% FBS for the cell migration assay with 20× magnification. Treatment with rHMGB1 (100 ng/mL) increases the ability of cells to migrate into the empty area and to repair the wound at all-time points (24 and 48 h) examined. Yellow arrows indicate migration into the cell free-space; (B) The ability of cells to migrate and cover the empty space was determined as a wound repair by percent of 0 h (T0). Data are mean ± SD of 3–5 independent experiments. * p < 0.001 vs. the control siRNA and blank control groups; (C) Expression of PCNA mRNA in the cells after addition of 300 nM RAGE siRNA or control siRNA and stimulation by 100 ng/mL rHMGB1 was quantified by real time PCR analysis and reported by normalized to GAPDH. Data are expressed as the mean ± SD of three independent determinations. * p < 0.001 vs. the rHMGB1 group.