Figure 1.

Overexpression of PCAF induced autophagy in HCC cells. PCAF expression was significantly increased by PCAF expressing plasmid in Huh7 cells at both the mRNA (a) and protein (b) level, compared with Huh7 Control cells. The results are summarized as relative band intensity of PCAF protein. The experiments were repeated at least three times. (c) Huh7 cells were transfected with plasmids expressing full-length PCAF mRNA or empty control plasmid, and Huh7 Control cells and Huh7 PCAF cells were treated with 5 mM 3-MA or 50 nM bafilomycin A1 for 24 h. Cell lysates were analyzed by immunoblotting for LC3 II and p62. In Huh7 cells, 3-MA decreased the expression of LC3 II and increased p62 expression, whereas the effect of bafilomycin A1 was opposite. The experiments were repeated at least three times. (d) The above-described cells were incubated with anti-LC3 antibody and were assessed by confocal microscopy. *P<0.05, **P<0.01