Figure 2.

Knockdown of PCAF decreased autophagy in HCC cells. (a) PCAF expression was significantly decreased by PCAF siRNA in Hep3B cells at both the mRNA and protein level, compared with Hep3B Scr siRNA cells. The experiments were repeated at least three times. (b) In Hep3B Scr siRNA cells, Hep3B PCAF siRNA cells, Hep3B Scr siRNA cells treated with bafilomycin A1 and Hep3B PCAF siRNA cells treated with bafilomycin A1, the expression of LC3 II and p62 was assessed western immunoblotting. The experiments were repeated at least three times. (c) The LC3 II puncta in above cells were demonstrated by the confocal microscopy. *P<0.05, **P<0.01