Figure 6.

PCAF inhibited Akt/mTOR signaling. (a) Huh7 PCAF cells, Huh7 Control cells, Hep3B PCAF siRNA cells and Hep3B Scr siRNA cells were analyzed for Akt/mTOR activity by western blotting assay. The experiments were repeated at least three times. Huh7 PCAF cells and Huh7 Control cells in presence or absence of 10 μM Akt1/2 inhibitor (b) and 10 nM rapamycin (c) were also analyzed for Akt/mTOR activity by western blotting assay. The experiments were repeated at least three times. (d) LC3 II puncta in the above-described cell lines were imaged by the confocal microscopy. *P<0.05, **P<0.01