Figure 7.

TRAIL reduces the expression of adipogenic transcription factors. SGBS cells were treated with TRAIL (30 ng/ml) in the absence or presence of the pan-caspase inhibitor zVAD.fmk (20 μM) for the first 4 days of adipogenic differentiation. RNA was isolated after 48, 72, 120, 168, and 240 h and qPCR analysis of the adipogenic transcription factors C/EBPβ (a), C/EBPδ (b), PPARγ (c), C/EBPα (d), and SREBP1C (e) was performed. The mRNA levels were normalized to the gene HPRT. Displayed are the means and S.E.M. of three independent experiments. (f) Protein was isolated after 7 and 10 days and the protein expression of C/EBPβ, C/EBPδ, PPARγ, C/EBPα, and SREBP1C was analyzed by western blot. α-Tubulin was used as a loading control. The positions of the molecular weight markers (kDa) are indicated. One representative out of three experiments performed is presented. Two-way ANOVA and Turkey's multiple comparison were used to test for statistical significance in (a–e). *P<0.05; **P<0.01; ***P<0.001, vehicle versus TRAIL