Figure 1.

The upregulation of miR-125b is correlated with MK determination and maturation. (a) CD34⁺ hematopoietic cells were differentiated to MKs by culture in a megakaryocytic differentiation medium. The proportion of CD41⁺/CD61⁺ cells is indicated. (b) Isolated PLT from CB samples highly express the surface markers CD61 and CD42. (c) Isolation of CD34⁺ hematopoietic cells and megakaryoblasts at different stages of development was performed by cell sorting based on the expression levels of surface markers. Morphological difference between different stages was shown by Wright–Giemsa staining. Over 100 cells from five random views were measured. The error bars represent standard deviation (S.D.). Dunnett's test T3 were used for statistical analysis. (d) MiR-125b expression varies in different MK differentiation stages and during each megakaryocytic induction time. MK-specific induction starts with primary human HSCs. Changes in miR-125b were evaluated by qPCR. Comparative miRNA real-time PCR was performed in triplicate, and the expression levels were normalized to U6 miRNA. The error bars represent S.D. All of the data are expressed as the mean±S.D. from three experiments. Cells were obtained from three different donors. Bonferroni's multiple comparison test was used for statistical analysis. ***P<0.001 and **P<0.01