Figure 3.

The C-terminal region of PKN2 is sufficient to enhance myoblast differentiation. (a) C2C12 cells were stably transfected with GST-tagged PKN2 and its deletion mutants, or control expression vectors (pcDNA). Lysates of these cell lines were immunoblotted with antibodies to GST to reveal the level of ectopic expression of PKN2 and PKN2 mutants. In addition, lysates were immunoblotted with antibodies to MHC, Myogenin and pan-Cadherin as a loading control. (b) C2C12 cells shown in a were cultured in DM for 2 days, fixed and immunostained with an antibody to MHC followed by DAPI staining to visualize nuclei. Size bar, 100 μm. (c) Quantification of myotube formation by cell lines shown in (b). Values represent means of triplicate determinations ±1 S.D. The experiment was repeated three times with similar results. Significant difference from control, *P<0.01, **P<0.05