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. 2016 Oct 13;7(10):e2421. doi: 10.1038/cddis.2016.305

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Pectolinarigenin inhibits tumor growth in a patient-derived osteosarcoma xenograft (PDX) animal model. (a) Representative images of patient-derived osteosarcoma grafts removed from mice after administration of pectolinarigenin for 28 days (left panel). Summary results of the PDX tumor volume in control group and pectolinarigenin-treated group (***P<0.001) (right panel). (b) PDX tumor weight in each group was measured (***P<0.001). (c) Patient-derived grafts were fixed and paraffin embedded. Four micrometer (4 μm) sections were analyzed by IHC using an anti-phospho-STAT3 (Y705) antibody. Scale bar, 100 μm. (d) PDX tumor was lysed and applied to immunoblotting with with indicated antibodies. Actin was used as a loading control