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. 2016 Oct 13;7(10):e2413. doi: 10.1038/cddis.2016.306

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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MiR-590-5p inhibits CRC angiogenesis. (a) Subcutaneous CRC xenografts were established in nude mice using SW480-scramble, SW480-miR-590-5p inhibitor, SW620-control, or SW620-miR-590-5p cells, respectively. Then these tumor sections were stained with anti-CD31 (upper panel) and anti-DIG (lower panel) antibodies. (b) Graphical representation of tumor volume monitored after 4 weeks. (c) The indicated CM were loaded onto CAM, and the representative pictures were presented. (d) The new blood vessel formation in CAM was quantified. (e) The CM from the indicated cells were used to treat HUVECs, and tube formation was photographed, and (f) quantified. *P<0.05, **P<0.01