Figure 6.

Acute and chronic nutritional stress accelerates trafficking of intracellular C3. (a) Western blotting of whole-liver lysates from wild-type and ob/ob fasted and refed mice. Proteins were separated by SDS-page under reducing conditions. An antibody against C3a was used. Short exposures (s.e.) and long exposures (l.e.) are shown. Arrows indicate endogenous pro-C3 and the alpha chain (α chain) of mature C3. Asterisk (*) indicate unspecific band. An antibody against p-IRE1α was used to assess ER stress. Liver lysates from C3 knockout mice (C3KO) were used as negative controls. (b) Model of C3 intracellular trafficking and secretion in conditions of fasting versus refeeding with high-sucrose diet or obesity