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METH and gp120 IIIb induce autophagy through mTOR, Beclin-1 and Atg5/7 pathways. SVGA cells were treated with 1 mM METH and 400 pM gp120 IIIb for 24 h and were then subjected to western blotting to measure the signaling proteins p-mTOR (a), Beclin-1 (b), Atg5 (c) and Atg7 (d). The results are shown as mean±S.E. from three independent experiments. Data quantified by AlphaEase FC software are shown at the bottom of each panel. *P<0.05
