Figure 1.

(a) Vascular response of phenylephrine-precontracted mice mesenteric arteries to increasing doses of BAG3 (0.006–12 μg/ml) (n=6), (b) and after 30 min pretreatment with L-NAME (300 μmol/L; n=6), *P<0.05, **P<0.01 versus BAG3 alone. (c) Representative immunoblot of mesenteric arteries, untreated, treated with acetylcholine (Ach) or with BAG3; right, Columns are the mean±S.D. of 5 independent experiments. *P<0.05. (d) Vascular response of phenylephrine-precontracted mice mesenteric arteries to increasing doses of BAG3 (0.006–12 μg/ml) in presence of PI3K inhibitor (wortmannin 10 μM, 1 h, (n=6), *P<0.05, **P<0.01 versus. BAG3 alone. (e) Representative immunoblot of mesenteric arteries, treated with acetylcholine (Ach), with BAG3 or with BAG3 plus wortmannin; Bottom, columns are the mean±S.D. of 5 independent experiments. (f) Representative immunoblot performed on HUVEC treated with different doses of BAG3, Acetylcholine (ACh) in presence or absence of wortmannin. (g) HUVEC viability at 24 h after treatment with different doses of BAG3 was assessed by the trypan blue exclusion test. The data presented are the mean±S.D. of three independent experiments