Figure 8.

DFO increased HO-1 expression by dampening p38MAPK pathway. (a) Western blot analysis was performed to evaluate the effect of DFO on RANKL-induced the phosphorylation of p65, p38, ERK and JNK. (b) Western blot analysis was performed to evaluate the effect of blocking p38, JNK, NF-κB and MEK on DFO-induced HO-1 expression. (c) qRT-PCR analysis was performed to evaluate the effect of blocking p38, JNK, NF-κB and MEK on DFO-induced HO-1 expression. **P<0.01. # versus control, ^#P<0.01. (d,e) Western blot analysis showed that SB203580 promoted DFO-induced HO-1 expression in a dose-dependent manner