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. 2016 Oct 27;7(10):e2442. doi: 10.1038/cddis.2016.346

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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EHF knockdown inhibits EMT process in gastric cancer cells. (a) The expression of E-cadherin and Vimentin was determined in the indicated cells by western blot analysis. GAPDH was used as loading control. (b) Immunofluorescence staining was then used to assess the expression of E-cadherin and Vimentin proteins in cells transfected with si-EHF-979 or si-NC. Red color represents target protein fluorescence and blue color represents Hoechst33342 staining for nuclei. Magnification for each set: × 400. Scale bars, 100 μm