Figure 7.

EHF regulates the expression of HER receptors and the activities of their downstream signaling pathways in gastric cancer cells. (a) qRT-PCR assay was used to evaluate mRNA expression of EHF and HER receptors in primary gastric cancers (n=30). Linear regression analysis was performed to assess the correlations between them. 18S rRNA was used as a normalized control. (b) qRT-PCR assay was performed to investigate the effect of EHF knockdown on the expression of HER receptors. Expression levels of these genes were normalized with 18S rRNA levels. Data were presented as mean±S.E. (c) The effect of EHF depletion on the expression of HER2-4 was determined in the indicated cells by western blot analysis. GAPDH was used as loading control. (d) Cells transfected with si-EHF-979 or si-NC were lysed and lysates were subjected to western blot analysis. The antibodies against phospho-Erk (p-Erk), total Erk (t-Erk), phospho-Akt (p-Akt) and total Akt (t-Akt) were used to determine the effect of EHF knockdown on the activities of the MAPK/Erk and PI3K/Akt cascades. GAPDH was used as a loading control