Figure 6.

WT Treg cells suppress Il2ra^−/−Tg T cells after bone marrow chimera. (a) Strategy of bone marrow chimera: 5 × 10⁵ T-cell depleted bone marrow cells from CD45.1 Foxp3^GFP mice were mixed with 5 × 10⁵ T-cell depleted bone marrow cells from CD45.2 Il2ra^−/−Tg or littermate control mice and transferred intravenously to lethally irradiated CD45.1 CD45.2 recipient mice. Mice were killed 10 weeks after bone marrow transfer. (b) Naive T cell (CD44⁻CD62L⁺) percentage of pLN CD4⁺ and CD8⁺ T cells derived from Il2ra^−/−Tg (n=3), Il2ra^−/− (n=4), Il2ra^+/−Tg (n=4) and Il2ra^+/− (n=4) bone marrow. (c) Treg percentage in total CD4⁺ T cells after chimerism of Il2ra^−/−Tg (n=5), Il2ra^−/− (n=6), Il2ra^+/−Tg (n=6) and Il2ra^+/− (n=6) with WT bone marrow. (d) Tfh percentage of CD4⁺ T cells, (e) GC B percentage of B cells and (f) plasma cell percentage of total cells derived from Il2ra^−/−Tg (n=5), Il2ra^−/− (n=6), Il2ra^+/−Tg (n=6) and Il2ra^+/− (n=6) bone marrow. (g) Tfr cell percentage of Tfh cells after chimerism of Il2ra^−/−Tg (n=4), Il2ra^−/− (n=5), Il2ra^+/−Tg (n=5) and Il2ra^+/− (n=5) with WT bone marrow. Data are shown in mean±S.E.M. **P<0.01. (Student's t test)