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. 2016 Dec 1;7:180. doi: 10.1186/s13287-016-0438-5

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — ADAMTS5 and IGFBP5 are inversely correlated with the expression of miR-140 and positively correlated with the tumor stage and metastasis of CRC. a qRT-PCR analysis of ADAMTS5 and IGFBP5 expressions in 60 pairs of CRC and adjacent nontumorous tissues. b Correlation of ADAMTS5 and IGFBP5 and miR-140 expression was analyzed by Spearman correlation test in CRC tissues. c Immunohistochemistry analysis of ADAMTS5 and IGFBP5 expressions in CRC tissues. 1, 2, 5 and 6: magnification × 20; and 3, 4, 7 and 8: magnification × 40; 1, 3, 5 and 7: primary sites of tumor tissue; and 2, 4, 6 and 8: noncancerous region of CRC. Scale bar = 100 μm. The results of immunohistochemistry were evaluated by the staining scores. *P < 0.05