Table 2.

Primers used for QPCR

Gene	Forward		Reverse		Amplicon	Tm (°C)
	Sequence	Position	Sequence	Position
ATF-3	TGCCTGCAGAAGGAGTCAGAG	494–514	CTTCCGGCGTCCGCCCGTTCT	650–630	157	56
CD68	AATTCACCTGGACCTGCTCTC	837–857	ACGCAGAAGGCAATGAGCACC	1004–984	168	54
C-FOS	CTGTCAACACACAGGACTTTT	262–282	CGCTCTGGTCTGCGATGGGGC	410–390	149	50
CGRP	AACCTTAGAAAGCAGCCCAGGCATG	102–126	GTGGGCACAAAGTTGTCCTTCACCA	442–418	246	59
COL18A1	AGGTAAGGATCTGGGCTA	3064–3081	CCTCGTTGTCCGTCCCG	3237–3221	174	50
COX-2	CGAGGACTGGGCCATGGAGTG	746–766	ACCTCTCCACCGATGACCTGA	864–844	119	57
DYN	GCCCCAGCAGGAAGGGTGATA	211–231	CAGGGACGAAATCAGGGGGTT	371–359	157	57
GAPDH	ACCATCTTCCAGGAGCGAGA	292–311	GGCGGAGATGATGACCCT	438–421	147	56
ID1	GTGAGCAAGGTGGAGATACTG	304–321	GCTGGAACACATGCCGCCTCG	482–462	179	52
IL-1β	GACCCCAAAAGATTAAGGATT	136–156	AAAGAAGGTGCTTGGGTCCTC	335–315	200	50
MCP-1	CCAGAAACCAGCCAACTCTC	25–44	CCGACTCATTGGGATCATCT	216–197	192	53
S100A8	GTGCAGAATAAAAATACCGAA	159–179	GCTGTCTTTATGAGCTGCCAC	281–261	123	48
SMAD6	ACTGGATCTGTCCGATTCTAC	873–893	CCGGAGCTCCCAGTACGCCAC	1023–1003	151	50
SMAD7	AGAGTCTCCCCCTCCTCCTTA	755–775	AAGAAGTTGGGAATCTGAAAG	899–879	142	48
SUB P	AGAGGAAATCGGTGCCAAC	2128–2107	TGCCCATTAATCCAAAGAACTG	1456–1474	152	52
TNF-α	CGGTCCCAACAAGGAGGAGAA	323–343	AGGAGGGCGTTGGCACGCTGG	502–482	180	50
TRPV1	TACTACAAGGGCCAGACAGCA	672–692	GGGGCAGCTCACCAAAGTAGA	834–814	163	55