Extended Data Table 2.
Data collection and refinement statistics.
| Cascade-dsDNA | Cascade-dsDNA (32-nt non-target spacer) | |
|---|---|---|
| Data collection | ||
| Space group | P2 2121 | P 2 21 21 |
| Cell dimensions | ||
| a, b, c (Å) | 92.98, 150.06,400.55 | 92.81,149.83,404.10 |
| α, β, γ (°) | 90, 90, 90 | 90, 90, 90 |
| Resolution (Å) | 50.0–2.45 (2.49−2.45) | 50.0–3.20 (3.26−3.21)* |
| Rmerge | 0.169 (1.288) | 0.265 (1.066) |
| Rpim | 0.050 (0.531) | 0.143 (0.667) |
| I/σI | 12.9 (1.1) | 5.8 (1.5) |
| Completeness (%) | 99.7 (97.2) | 96.8 (84.1) |
| Redundancy | 12.1 (6.5) | 3.6 (3.0) |
| CC(l/2) | 0.997 (0.496) | 0.967 (0.422) |
| Refinement | ||
| Resolution (Å) | 50.0–2.45 | 50.0–3.21 |
| No. reflections | 205,381 | 90,215 |
| Rwork/Rfree | 0.2058/0.2327 | 0.2087/0.2470 |
| No. atoms | ||
| Macromolecule | 27896 | 27237 |
| Ligand/ion | 1(Zn) | 1(Zn) |
| Water | 1051 | 0 |
| B-factors | ||
| Macromolecule | 53.60 | 62.10 |
| Ligand/ion | 51.90 | 61.50 |
| Water | 45.90 | N/A |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.013 | 0.018 |
| Bond angles (°) | 0.840 | 1.010 |
*
One crystal was used for each structure.
*
Values in parentheses are for highest-resolution shell.