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. 2016 Dec 1;9:262. doi: 10.1186/s13068-016-0675-y

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Schematic of the construction of ribosome binding site library (RBSL) of PPP enzymes. The seven PPP enzymes locate dispersedly among E. coli MG1655 chromosome. A constitutive promoter M1-93 was initially used to replace native promoter of PPP enzymes. Next, seven degenerate nucleotides (RNNNNNN) were introduced into the RBS region of M1-93 promoter before ATG start codon to obtain the RBSL