Fig. 1.

Levels of anti-VHSV antibodies in immunized muskellunge. Levels of circulating anti-VHSV antibodies of 27 muskellunge for 10 weeks vaccinated with 10 μg of the pVHSivb-G indicated by indirect ELISA optical density (OD₄₉₀) values. The predicted relationships from 0.50, 0.75, and 0.90 quantile regression models was fit to the OD₄₉₀ values as a function of time since vaccination. The dashed lines represent ±1 SE of the predicted relationships. The cut off value was estimated to be 0.163, above which a sample was considered positive