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. 2016 Dec 1;15:205. doi: 10.1186/s12934-016-0605-5

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Illustration of modularized assembly strategy. a Editing plasmid modular parts with optimized 4 nt TypeIIS resection enzyme linkers; b maps of fixed backbone modular part1 and part2; c map of assembled pRed_Cas9_recA_Δpoxb300 from modular designed parts