Figure 2.

Accumulation of autophagic vacuoles positively correlated with the incubated level of H₂S. (A) Representative TEM photographs of pancreatic tissues harvested from the rats as described in Figure 1A. The percentage of autophagic vacuoles per cytoplasm area was totalled, bar: 2 μm. (B) Representative IHC photographs and corresponding IODs of LC3 expression in pancreatic tissues harvested from the rats as described in Figure 1A, bar: 50 μm. (C and D) Representative immunoblot images of LC3 expression (top) and quantitations of LC3 conversion (bottom) in pancreatic tissues (C) harvested from the rats as described in Figure 1A and in AR42J cells (D) that were subjected to control, AP, AP+NaHS or AP+PAG for 3 hrs since AP induction. β‐actin was used as the protein loading control. Data were presented as mean ± S.D. (n = 3). *P < 0.05 versus sham or control, ^P < 0.05 versus AP.