Figure 4.

Knockdown of MITOL sensitizes cardiomyocytes to mitochondrial fission and apoptosis. (A) Immunoblot shows MITOL knockdown in HL‐1 cells. (B and C) Knockdown of MITOL sensitizes cells to undergo mitochondrial fission. HL‐1 cells were exposed to H₂O₂. (B) It shows mitochondrial morphology, bar = 2 μm. (C) It shows cells with mitochondrial fission; *P < 0.05 versus H₂O₂ alone. (D) Immunoblot shows cleaved‐PARP. (E) Knockdown of MITOL sensitizes cells to undergo apoptosis. Apoptosis was analysed by TUNEL assay. *P < 0.05 versus H₂O₂ alone. (F) Knockdown of MITOL sensitizes cells to undergo DNA fragmentation. DNA fragments were analysed using the cell death detection ELISA. *P < 0.05 versus H₂O₂ alone. Data were expressed as the mean ± S.E.M. of three independent experiments.