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. 2016 Jul 15;20(12):2289–2298. doi: 10.1111/jcmm.12919

Figure 3.

Figure 3

MS275 regulates TGF‐β1‐mediated ECM proteins and E‐cadherin in HK2 cells. (A) HK2 cells were incubated with increasing concentrations of MS275 (0, 0.1, 1 and 10 μM) for 24 hrs and cell viability was determined. (B) HK2 cells were co‐treated with TGF‐β1 (5 ng/ml) and MS275 (1 μM). Proteins from cell lysates were subjected to SDS‐PAGE. Representative Western blot images are shown. β‐Actin was used as the loading control. (C–F) Quantification was performed by densitometry for at least four independent experiments. (G) Representative immunofluorescence images for E‐cadherin in HK2 cells. Immunofluorescence staining was performed using anti‐E‐cadherin antibody and cell nuclei were stained with DAPI. Merged images are shown. Scale bar represents 50 μm. **P < 0.01 and ***P < 0.001 compared with untreated cells. # P < 0.05 and ### P < 0.001 compared with TGF‐β1 treated cells. NS, not significant.