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. 2016 Jul 15;20(12):2289–2298. doi: 10.1111/jcmm.12919

Figure 4.

Figure 4

PCI34051 did not affect TGF‐β1‐mediated EMT markers in HK2 cells. (A) Cytotoxicity of PCI34051 in HK2 cells. (B) HK2 cells were co‐treated with TGF‐β1 (5 ng/ml) and PCI34051 (5 μM) for 24 hrs. Western blot analysis was performed using collagen type I (collagen 1), fibronectin, N‐cadherin, and E‐cadherin antibodies. β‐Actin was used as the loading control. (C–F) Quantification was performed by densitometry for at least four independent experiments. **P < 0.01 and ***P < 0.001 compared with untreated cells. # P < 0.05 and ### P < 0.001 compared with TGF‐β1 treated cells. NS, not significant.