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. 2016 Jul 15;20(12):2289–2298. doi: 10.1111/jcmm.12919

Figure 5.

Figure 5

LMK235 did not affect TGF‐β1‐mediated ECM proteins and EMT markers in HK2 cells. (A) Cytotoxicity of LMK235 in HK2 cells. (B) HK2 cells were co‐treated with TGF‐β1 and LMK235 for 24 hrs. Western blot analysis was performed using collagen I, fibronectin, N‐cadherin, and E‐cadherin antibodies. β‐Actin was used as the loading control. (C–F) Quantification was performed by densitometry for at least four independent experiments. *P < 0.05 and ***P < 0.001 compared with untreated cells. ## P < 0.01 and ### P < 0.001 compared with TGF‐b1 treated cells. NS, not significant.