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. 2016 Jul 15;20(12):2289–2298. doi: 10.1111/jcmm.12919

Figure 6.

Figure 6

Knockdown of HDAC8 blocks ECM proteins and EMT markers induced by TGF‐β1. (A) HK2 cells were transfected with siRNA against class I HDACs (HDAC1, 2, 3 and 8) or siRNA control and then incubated with TGF‐β1 for 24 hrs. ***P < 0.001 compared with siRNA control. (B) Representative immunoblots for ECM proteins, EMT markers and class I HDACs. (C–F) Quantification was performed by densitometry for at least six independent experiments. ***P < 0.001 compared with siRNA control. # P < 0.05, ## P < 0.01 and ### P < 0.001 compared with TGF‐β1‐treated siRNA control cells.