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. 2016 Sep 14;20(12):2384–2404. doi: 10.1111/jcmm.12932

Figure 1.

Figure 1

Flow cytometry and multipotency analysis. Flow cytometry dot plots representative for equine adipose derived mesenchymal stem cells (ASCs) from control (A) and EMS (B) group. Isolated cells are characterized by the presence of CD44, CD90, CD105 and CD45. ASCs showed the lack of expression CD45 surface markers (A, B, F). Flow cytometry analysis determined the percentage of specific markers in total analysed ASCs. Quantification of markers revealed significantly higher expression of CD44 in EMS group (P < 0.001, C). We did not observe differences in the expression of CD90 (D), CD105 (E) and CD45 (F), respectively. Representative images from tri‐lineage assay of ASCs differentiation (G). Cells cultured in control medium served as a control to monitor effectiveness of differentiation process (I, V). Osteogenically differentiated cells in monolayer stained with Alizarin Red (II, VI), chondrogenically differentiated cells using Safranin O (III, VII) and adipogenically differentiated cells stained using LipidTOX (IV, VIII). Magnification ×100, scale bars: 250 μm. Results expressed as mean ± S.D., ***P < 0.001.