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Optimization of the reaction conditions: (A) temperature (50 nM DNA probe, 50 μM dNTP, 20 U/mL KF polymerase); (B) concentration of dNTP (50 nM DNA probe and 20 U/mL KF polymerase); (C) concentration of KF polymerase (50 nM DNA probe and 60 μM dNTP). Error bars show the standard deviation of three experiments.
