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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Mol Microbiol. 2016 Jun 16;101(5):856–878. doi: 10.1111/mmi.13426

Figure 1. Genomic organization and generation of the oxyR gene deletion mutant of the F. tularensis LVS.

Figure 1

(A) The genomic organization of the oxyR gene of the F. tularensis LVS and its comparison with the oxyR gene from the indicated bacterial pathogens. (B) Multiplex colony PCR using oxyR gene-specific primers and sodB gene primers as internal controls. Amplification of the sodB gene confirmed the presence of the DNA template in the reaction, whereas the absence of the oxyR gene product in the ΔoxyR mutant confirmed the deletion of the oxyR gene.