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BrdU immunostaining. Injection of CDK9-targeting morpholino or exposure to Flavopiridol reduced significantly the number of BrdU positive nuclei compared to controls. Brdu figures were counted in the tail region as shown in these images and reported in the scatter graph. At least n = 20 embryo per group; data were statistically analyzed by student t-test; ** = ≤0.01, *** = ≤0.001.
