Figure 4.

ChIP Analyses of Chromosome Occupancy by Replication Initiation and DNA Repair Factors Modulated by HU Treatment

(A–D) ChIP analyses of binding of the Orc1-3 and WhiP proteins to the three replication origins (A–C) and distal control locus SSO2847 (D) in cells treated with 0 or 10 mM HU for 7 hr. ChIP reactions were performed in triplicate, and data are expressed as fractional recovery of the total input material.

(E–H) Occupancy of the indicated genomic loci by MCM (E), Alba (F), Hel308 (G), and RadA (H) as adjudged by ChIP from untreated cells or cells treated with 5 or 10 mM HU for 4 hr.

Mean values of the triplicate repeats are shown, and error bars indicate the SD of the data.