CYP92C5 Is Inactive in the Transposon Insertion Mutant MuIII.
(A) Volatiles found in of leaves of the lines B73 and transposon insertion mutant MuIII after induction with the indanone elicitor. The volatiles were collected with a polydimethylsiloxane-coated SPME fiber and analyzed by GC-MS. The major terpene compounds that were identified are 1-DMNT, 2-(E)-nerolidol, and 3-TMTT.
(B) Levels of transcripts encoding CYP92C5 in leaves of the lines B73 and MuIII. Leaves of 14-d-old plants were wounded and treated with indanone derivate for 24 h. Transcript abundance was determined relative to the APT1 reference gene. One-way ANOVA was used to calculate the significance of three plants per treatment. Error bars represent se.