Figure 7.

TPS2 Is Inactive in the Inbred Line Tzi8.

(A) Volatiles found in leaves of the inbred lines B73 and Tzi8 after induction with indanone elicitor. The volatiles were analyzed by GC-MS. The major terpene compounds were identified as 1-linalool, 2-DMNT, 3-(E)-nerolidol, 4-TMTT, 5-(E)-α-bergamotene, and 6-(E)-β-farnesene.

(B) TPS2 alleles from B73 and Tzi8 were expressed in E. coli, and protein was extracted, partially purified, and incubated with GDP/FDP/GGDP substrates. The resulting terpene products were collected with a polydimethylsiloxane-coated SPME fiber and analyzed by GC-MS.

(C) Levels of transcripts encoding TPS2 in leaves of the lines Tzi8 and B73. Leaves of 14-d-old plants were wounded and treated with indanone derivative (Elicitor) for 24 h or left undamaged (Control). Transcript abundance was determined relative to the reference gene encoding APT1. One-way ANOVA was used to calculate the significance of three plants per treatment. Error bars represent se. Different letters denote significant differences at P < 0.01.