Figure 2.

The late2-1D Mutation Affects Graft-Transmissible Flowering Signals and Induction of Flowering Genes.

(A) Node of flower initiation and growth termination for graft combinations of wild-type NGB5839 (WT) and the late2-1D mutant, in comparison to intact and self-grafted controls. For each graft combination, the genotypes of scion (top) and stock (bottom) are shown, separated by a horizontal line. Six-day-old shoots (scions) were excised above the cotyledons and the epicotyl grafted onto the uppermost internode of 3-week-old stocks. Values represent mean ± se for n = 10 to 20 plants grown in LD.

(B) Gene expression in the wild type (NGB5839) and the late2-1D mutant during development under continuous light. Relative transcript levels were determined in dissected shoot apices or the second-uppermost fully expanded leaf. Mean values ± se are shown for n = 2 to 3 biological replicates, each consisting of pooled material from two plants. Developing floral buds were first visible in the wild type 27 d after sowing and in late2-1D 40 d after sowing (broken lines).