Figure 6.

The R450W Mutation in late2-1D Affects the Function of the CDFc1 Protein.

(A) Yeast two-hybrid analysis of the interaction between pea FKF1 and the wild-type and R450W mutant form of CDFc1. The previously characterized interaction between Arabidopsis FKF1 and CDF1 proteins (Imaizumi et al., 2005) was included as a positive control. Each panel shows bait/prey interactions in both orientations, either FKF1/CDF (left) or CDF/FKF1 (right). Each clone is shown after 4 d of growth at 30°C on selective medium (with 3-amino-1,2,4-triazole added; -His+3-AT) and nonselective medium (+His). Negative controls are shown in Supplemental Figure 5.

(B) Representative plants from three independent Arabidopsis lines (Columbia accession) transformed either with wild-type or R450W mutant forms of pea CDFc1, grown under LD conditions.

(C) Relative expression levels at ZT 15.45 of PsCDFc1, AtCO, and AtFT and leaf number at flowering in transgenic Arabidopsis lines. Labels 1 to 3 denote independent transformants. Values represent mean ± se for n = 2 biological replicates (expression data) and n = 6 to 10 plants (leaf number).