Figure 9.

Classification of Terpene Synthase-Like Proteins Using Sequence Similarity Networks of Related Proteins.

Group I: Diterpene synthase-like proteins from M. polymorpha (MpDTPS; pink squares) in the context of their closest relatives from the SFLD, the terpene synthase-like 1 C-terminal domain subgroup, at a –log (E-value) cutoff of 110, colored by genus. Selaginella sequences are represented as magenta triangles. Group II: MpMTPSL1-5 and -8 from M. polymorpha (magenta squares) in the context of their closest relatives from the SFLD, the terpene cyclase-like 2 subgroup, at a –log (E-value) cutoff of 19, colored by Swiss-Prot annotation. S. moellendorffii sequences are represented as triangles. Note that the M. polymorpha sequences connect to other fungal and bacterial genes via pentalenene synthase. Group III: MpMTPSL6-7 and -9 from M. polymorpha (green squares) in the context of their closest relatives from the SFLD trichodiene synthase-like subgroup at a –log (E-value) cutoff of 17, colored by phylum. The sequences chosen for construction of phylogenetic trees are identified by yellow triangle nodes and represent synthases that have been functionally characterized and are evenly dispersed across the groups making up the networks.