Table 1. Proteins Identified by LC-MS/MS after Immunoprecipitation of P20 IgG from BaMV and satBaMV Coinfected N. benthamiana.
Band | Accession | Protein Name | Mass (kD) | Score |
---|---|---|---|---|
1 | ATCG00490.1 | RBCL ribulose-bisphosphate carboxylase | 53.4 | 59 |
2 | AT4G25630.1 | FIB2, ATFIB2 fibrillarin 2 | 33.8 | 42 |
2 | *gi|345134903|dbj|BAK64672.1| | *BaMV TGBp1 | 27.7 | 43 |
3 | AT5G01530.1 | Light-harvesting complex PSII | 25.2 | 130 |
3 | *gi|2407623|gb|AAB70566.1| | *BaMV CP | 25.5 | 818 |
4 | AT3G16640.1 | TCTP translationally controlled tumor protein | 21.3 | 55 |
4 | *gi|37782235|gb|AAP31339.1| | *SatBaMV P20 | 19.9 | 26 |
5 | AT1G77300.1 | Histone H3 | 17.0 | 25 |
6 | ATCG00580.1 | PSBE PSII reaction center protein | 10.0 | 36 |
Asterisks indicate BaMV- or satBaMV-encoded proteins. Score is parameter characterizing identification reliability of a certain protein. In general, at score value > 23, identification is considered as reliable (P < 0.05).