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. 2016 Sep 30;28(10):2493–2509. doi: 10.1105/tpc.16.00131

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© 2016 American Society of Plant Biologists. All rights reserved.

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Figure 4. — HT1(A109V) Is an Active Kinase and HT1(A109V) Activity Is Not Inhibited by MPK12 as Efficiently as the Activity of HT1.

(A) HT1 and HT1(A109V) autophosphorylate and phosphorylate casein with similar activity. Coomassie Brilliant Blue G 250 staining (G250), immunoblot (IB), and autoradiograph are shown.

(B) and (C) HT1-induced autophosphorylation and casein phosphorylation are inhibited by MPK12, and the inhibition is decreased in HT1(A109V). Autoradiographs and Coomassie Brilliant Blue G 250 staining are shown.

(D) Quantification of MPK12-induced inhibition of HT1 and HT1(A109V) activity. Average of three experiments ± se is shown.