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. 2016 Sep 30;28(10):2493–2509. doi: 10.1105/tpc.16.00131

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© 2016 American Society of Plant Biologists. All rights reserved.

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Figure 5. — HT1 Inhibits OST1- and GHR1-Activated SLAC1 Anion Currents in Oocytes and MPK12 Counteracts HT1 but Not HT1(A109V)-Induced Downregulation of SLAC1 Activity in Oocytes.

(A) HT1 inhibits OST1-induced SLAC1 activation in oocytes.

(B) HT1 inhibits GHR1-induced SLAC1 activation in oocytes.

(C) MPK12 releases the inhibition of GHR1-induced SLAC1 activation in oocytes caused by HT1, but not by HT1(A109V). In (A) to (C), average currents ± se at −140 mV are shown. Statistically significantly different groups are denoted with different letters (ANOVA + Tukey unequal N HSD post hoc test). Sample size was 10 to 17 in (A), 5 to 11 in (B), and 8 to 12 in (C).

(D) Current-voltage relationship curves for the oocytes presented in (C). Split YFP (BiFC) was used in all cases of SLAC1 and OST1 or SLAC1 and GHR1 coexpression and is indicated by -YN or-YC in the names of respective proteins. Replicates for the experiments are shown in Supplemental Figure 14.