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. 2016 Oct 3;28(10):2528–2544. doi: 10.1105/tpc.16.00359

Figure 2.

Figure 2.

sGFP:ABCG25-Positive Endosomes Are Transported to the Lytic Vacuole via the PVC in Normal Growth Conditions.

(A) to (D) Colocalization of sGFP:ABCG25 with ARA7:RFP, but not with VHA-a1:RFP, in root tip cells. Transgenic plants harboring sGFP:ABCG25 together with VHA-a1:RFP (A) or ARA7:RFP (C) were examined for colocalization of sGFP:ABCG25 with VHA-a1 or ARA7 using a CLSM. Green, sGFP:ABCG25; red, ARA7:RFP or VHA-a1:RFP. Bars = 10 µm.

(B) and (D) The extent of the colocalization of green and red fluorescent signals was analyzed by ImageJ to obtain Pearson-Spearman correlation coefficients. The resulting scatterplots are shown along with rp and rs values. The level of colocalization ranged from +1 (complete colocalization) to −1 (no correlation). Analysis of the colocalization between sGFP:ABCG25 and VHA-a1, or between sGFP:ABCG25 and ARA7, was undertaken using 51 and 44 cells, respectively.

(E) Enlargement of sGFP:ABCG25-positive endosomes by Wortmannin treatment. sGFP:ABCG25 plants were treated with Wortmannin (33 µM) for 1.5 h. Bars = 10 µm.