Figure 5.

ABA-Induced Recycling of sGFP:ABCG25 from Endosomes to the PM Is Defective in ABA-Insensitive Mutant Plants.

(A) The levels of PM-localized sGFP:ABCG25 in QC3 mutant plants. Wild-type and QC3 seedlings harboring sGFP:ABCG25 were grown on 0.5× MS plates for 5 d, and GFP signals were examined in root tip cells. Bars = 10 µm.

(B) The effect of exogenously applied ABA on the recycling of sGFP:ABCG25 in QC3 plants. Five-day-old seedlings that had been treated with 50 µM BFA for 1 h were transferred to 0.5× MS liquid medium containing DMSO or 10 µM ABA. The disappearance of sGFP:ABCG25 from the BFA body was measured at the indicated time points. B, BFA body. Bars = 10 µm.