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Analysis of the Enzymatic Activity of ALD1 and SARD4 in E. coli.
(A) to (D) Extracted ion chromatograms for P2C (m/z 128.070, dotted lines) and Pip (m/z 130.086, solid lines). For the analysis, E. coli cultures expressing ALD1 (A), SARD4 (B), ALD1 and SARD4 together (C), or the two corresponding empty vectors (D) were used. Similar results were obtained in two independent experiments.
(E) Proposed scheme for the Pip biosynthesis pathway from lysine.
