Table 1. Kinetic Parameters of the At5g32470, GRMZM2G148896, and GRMZM2G078283 TenA-HAD Proteins with ThMP as Substrate.

Enzyme	Km (μM)	kcat (s−1)	kcat/Km (s−1 M−1)
At5g32470	1.86 ± 0.37	13.5 ± 0.8	7.25 × 106
GRMZM2G148896	3.61 ± 0.68	16.9 ± 1.3	4.68 × 106
GRMZM2G078283	3.20 ± 1.04	8.2 ± 1.0	2.57 × 106

All proteins were truncated to remove predicted targeting sequences. Assays contained 50 mM Tris-HCl buffer, pH 7.5, 10 mM MgCl₂, 10% glycerol, and 1 mg/mL BSA. Data are means and se for three independent determinations.