Extended Data Figure 2.
a-b, The virulence of the avrE−/hopM1− mutant is insensitive to humidity settings. a, Col-0 plants were syringe-infiltrated with indicated bacteria at 2×105 cfu/ml. Inoculated plants were kept under high (~95%) humidity, and pictures were taken 24 h post infiltration. b, Col-0 plants were syringe-infiltrated with Pst DC3000, the avrE− mutant, the hopM1− mutant or the avrE−/hopM1− mutant at 2×105 cfu/ml. Inoculated plants were kept under high (~95%) or low (20-40%) humidity. Pictures were taken 3 days post inoculation. Images were representative of leaves from more than four plants. c-d, The 6xHis:HopM1 transgenic plants were infiltrated with 0.1 nM DEX, the avrE−/hopM1− mutant (at 1×105 cfu/ml) or both. H2O was infiltrated as control. Infiltrated plants were kept at high humidity (~95%). Leaf pictures were taken 24 h post infiltration (c) and bacterial populations were determined 3 days post infiltration (d). * indicates a significant difference, as determined by Student’s t-test; (two-tailed); ***, p=1.03×10−5. n=6 technical replicates from there independent experiments (n=2 in each experiment); error bars, mean±s.d.