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. 2016 Dec 2;10(12):e0005142. doi: 10.1371/journal.pntd.0005142

Table 1. Oligonucleotide sequences of primers and probes used for real-time PCRs and conventional PCRs in this study.

Target Name Primers (5’-3’) and probes Source
Pediculus humanus
cytochrome b Duplex A-D F_ GATGTAAATAGAGGGTGGTT This study
R_ GAAATTCCTGAAAATCAAAC
FAM-CATTCTTGTCTACGTTCATATTTGG-TAMRA
VIC-TATTCTTGTCTACGTTCATGTTTGA-TAMRA
Duplex B-C F_ TTAGAGCGMTTRTTTACCC This study
R_ AYAAACACACAAAAMCTCCT
FAM-GAGCTGGATAGTGATAAGGTTTAT-MGB
VIC-CTTGCCGTTTATTTTGTTGGGGTTT-TAMRA
Cytb F_GAGCGACTGTAATTACTAATC [31]
R_CAACAAAATTATCCGGGTCC
Rickettsia spp.
citrate synthase (gltA) RKNDO3 F_GTGAATGAAAGATTACACTATTTAT [32]
R_GTATCTTAGCAATCATTCTAATAGC
FAM-CTATTATGCTTGCGGCTGTCGGTTC-TAMRA
Acinetobacter spp.
RNA polymerase β subunit gene rpoB F_TACTCATATACCGAAAAGAAACGG [18]
R_GGYTTACCAAGRCTATACTCAAC
FAM-CGCGAAGATATCGGTCTSCAAGC-TAMRA
rpoB (zone1) F_TAYCGYAAAGAYTTGAAAGAAG [33]
R_CMACACCYTTGTTMCCRTGA
Rickettsia prowazekii
rOmpB gene ompB F_AATGCTCTTGCAGCTGGTTCT [34]
R_TCGAGTGCTAATATTTTTGAAGCA
FAM-CGGTGGTGTTAATGCTGCGTTACAACA-TAMRA
Yersinia pestis
PLA F_ATG GAG CTT ATA CCG GAA AC [34]
R_GCG ATA CTG GCC TGC AAG
FAM-TCCCGAAAGGAGTGCGGGTAATAGG-TAMRA
Borrelia spp.
16S ribosomal RNA Bor16S F_AGCCTTTAAAGCTTCGCTTGTAG [35]
R_GCCTCCCGTAGGAGTCTGG
FAM-CCGGCCTGAGAGGGTGAACGG-TAMRA
flagellin gene flab F_GCTGAAGAGCTTGGAATGCAACC [36]
R_TGATCAGTTATCATTCTAATAGCA
Bartonella spp.
Internal transcribed spacer 16S-23S BartoITS2 F_GATGCCGGGGAAGGTTTTC [18]
R_GCCTGGGAGGACTTGAACCT
FAM-GCGCGCGCTTGATAAGCGTG-TAMRA
Anaplasma spp.
23S ribosomal RNA TtAna F_TGACAGCGTACCTTTTGCAT [37]
R_TGGAGGACCGAACCTGTTAC
FAM-GGATTAGACCCGAAACCAAG-TAMRA